There is no mention of what happened to her at all in the movie, other then the facts is that Disney likes to present movies that are a single parent story, or just get rid of them all together for the main child character can have some sort of adventure that would not happen if both parents were around. But I don't remember there ever being a mention of her, or where she is at so one could guess is that she could have died, or divorced Goofy. But your guess is as good as mine as to what happened to her.
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